State of the art reproductive technologies,

 compassionate patient care.

201 Pennsylvania Pkwy Suite 205

Indianapolis, IN 46280

telephone: 317-817-1147

fax: 317-817-1316

The Center for Reproductive Biology of Indiana logo

At The Center for Reproductive Biology of Indiana we have a state-of-the art IVF lab that is here to help you accomplish your dreams of conceiving a child. The lab is inspected and accredited by The Joint Commission (TJC) every two years, in accordance with Clinical Laboratory Improvement Amendments (CLIA) standards. It is registered with the FDA and is periodically inspected. The lab also voluntarily complies with the standards of the American Society for Reproductive Medicine (ASRM).


We regularly perform procedures such as:


• Egg retrieval for IVF

• Sperm prep for IUI

• In-vitro fertilization, IVF

• Assisted Hatching

• Intracytoplasmic Sperm Injection (ICSI)


Learn more about the staff at CRBI


What Happens in the IVF Laboratory?


Day 0 (Day of Oocyte Retrieval):

• The semen sample is collected, processed and motile sperm are isolated.

• The oocytes (eggs) are collected and allowed to “rest” in the incubator. Approximately 2-6 hours later:

• ICSI (Intracytoplasmic Sperm Injection)- Each mature oocyte will be injected with one motile sperm.




•  Insemination or “standard in vitro fertilization”- A specific number of sperm are added to the dish    and allowed to incubate with oocytes overnight.


Day 1: Fertilization Check

• In order for embryos to be normal, there must be two pronuclei  present (one from the oocyte and one from the sperm). If there is no pronucleus, the oocyte is considered “unfertilized”. If there are more than two present, the embryo is considered “polyploid”, resulting from an abnormally fertilized egg.


 Normally Fertilized Embryo

(pronuclei are the faint circles in center)



Day 3:

• Day 3 Transfer:

• Generally the embryos will be “hatched”, meaning that a small hole will be made in the zona pellucida (outer shell of the embryo). If there are remaining embryos for this patient post-transfer, they may be cultured until Day 5. They would be moved to a new culture medium.


• Day 3 Check

• If the patient is scheduled to have a Day 5 transfer, the embryos will be examined, evaluated, and moved to a new culture medium. On Day 3 of development, embryos are expected to be at approximately the 8 cell stage as shown in picture below.


• If Pre-implantation Genetic Diagnosis (PGD) is planned, the embryos will be biopsied on Day 3 and the samples will be sent by courier to a genetics lab for the appropriate testing. During biopsy, the embryo is hatched and one cell is removed for diagnosis.

8-cell Embryo


• Day 5

• On Day 5 of development, the embryos will have reached the Morula to Blastocyst stages (see below). The embryos will be graded on the morning of the transfer.  The patient and physician will discuss the number of embryos to be transferred.

• Remaining embryos that meet criteria will be cryopreserved if the patient requests. The embryo must be advanced to morula or blastocyst stage and be of good quality to be cryopreserved.

• Slower growing embryos may be cultured for an additional day (Day 6) to determine if they are eligible for cryopreservation.








The Lab

© 2016 The Center for Reproductive Biology of Indiana  All Rights Reserved.


Site designed by: Five Stones Marketing, LLC